ANTIBACTERIAL ACTIVITY OF Bacillus sp ISOLATED FROM LIWA BOTANICAL GARDEN SOIL AGAINST Dickeya sp.

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Published: Nov 28, 2024

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Kusuma Handayani
Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung
Lisa Maryati
Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung
Wawan A. Setiawan
Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung
M. Kanedi
Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung
Andi Setiawan
Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Abstract

The soil Bacillus group has been reported to produce antibacterial compounds. One of the plant pathogenic bacteria is Dickeya sp. which causes A strain of Bacillus bacteria from the soil of the Liwa Botanical Garden (KRL) has been successfully found and showed antibacterial activity against Dickeya sp. The Bacillus strain has been characterized as Bacillus vallismortis RKB1 strain through 16S rRNA phylogenetic analysis. This study aims to determine the activity of antibacterial compounds of Bacillus from KRL soil against Dickeya sp. Crude extract produced from liquid culture of Bacillus vallismortis RKB1 on antibacterial production medium showed antibacterial activity against Dickeya sp. Separation of compounds from crude extract by open column chromatography produced 8 fractions and there were 3 active fractions that showed inhibition against Dickeya sp. in the antibacterial activity test through agar disc diffusion. Fraction 54 showed the greatest inhibition and was classified as strong with an inhibition zone of 13.5 mm at a stock concentration of 5000 μg/ml. The fraction has a Minimum Inhibitory Level (KHM) at a concentration of 500 μg/ml based on turbidity value and resazurin staining through liquid microdilution. The identification results of Thin Layer Chromatography (KLT) on the active fraction with eluent n-hexanaana: ethyl acetate (7:3) showed a polar complex .This indicates that the compounds in the active fraction contain peptide compounds.

Article Details

Issue
ICEAST 2024
Section
Articles
Author Biographies

Kusuma Handayani, Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Lisa Maryati, Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Wawan A. Setiawan, Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

M. Kanedi, Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Biology Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Andi Setiawan, Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Lampung

Chemistry Department, Faculty of Mathematics and Natural Sciences, University of Lampung

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