Characterization of Pigmented Bacillus Species Isolated from Aquatic Environment

Authors

  • Saad Sabah Fakhry Ministry of Science and Technology
  • Ahmed Ibrahim Jessim Ministry of Science and Technology
  • Manal Abdulateef Hasan Ministry of Science and Technology

DOI:

https://doi.org/10.24853/jasat.4.2.51-62

Keywords:

Characterization Bacillus Isolated Pigment

Abstract

The genus of Bacillus includes several group of genetically and phenotypically different. Some species of spore-forming bacteria are pigmented, although the role of these pigments has not been fully elucidated. This study aimed to isolate a new species of Bacillus species and characterize both pigmented and pigment-producing bacteria product. For this purpose, samples were used water to isolate spore-forming bacteria and verify the presence of pigmentation. All bacteria found pegmatite were characterized at the species level and one of those selected for further studies. The physiological and biochemical analysis carried out revealed that: I) the product is carotenoid pigment, II) its synthesis takes place in stationary phase of growth. These features have allowed for us to assume that the carotenoid is produced under conditions of cellular stress and probably involved in cellular adaptation to some environmental conditions are not optimal for the cell.     

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References

Manzo, N., DiLuccia, B., Isticato, R., D’Apuzzo, E., De Felice, M., Ricca, E. 2013. Pigmentation and Sporulation are alternative cell fates in Bacillus pumilus SF214. PLoS ONE 8(4): e62093. https://doi.org/10.1371/journal.pone.0062093

Nicholson, W.L., Fajardo-Cavazos, P., Rebril, R., Slieman, T.A., Riesenman, P.J., Law, F., Xue, Y. 2002. Bacterial endospores and their significance in stress resistance. Antonie Van Leeuwenhoek. 81 (1-4): 27-32.

Sella, Sandra R.B.R., Vandenberghe, Luciana P.S., Soccol, C.R. 2014. Life cycle and spore resistance of spore –forming bacillus atrophaeus. Microbiological Research. 169 (12): 931-939

Tocheva, E.I., Matson, W.G., Morris, D.M., Moussavi, F., Leadbetter, J.R., Jensen, G.J. 2011. Peptidoglycan Remodeling and Conversion of an Inner Membrane into an Outer Membrane During Sporulation. National Institute of Health. 146 (5): 799-812

Checinska, A., Burbank, M., Paszczynski, A.J. 2012. Protection of Bacillus pumilus Spores by Catalases. Applied and Environmental Microbiology. 78 (18): 6413– 6422

Siefert, J.L., Larios-Sanz, M., Nakamura, L.K., Ralph A. Slepecky, R.A., Paul, J.H., Moore, E.R.B., Fox, G.E., Peter Jurtshuk, Jr. 2000. Phylogeny of Marine Bacillus Isolates from the Gulf of Mexico. Current Microbiology. 41: 84-88

Setlow, P. 2014. Germination of Spores of Bacillus Species: what we know and do not know. Journal of Bacteriology. 196(7): 1297–1305.

Banerjee, D., Chatterjee, S., Banerjee, U.C., Guha, A.K., Ray, L. 2011. Green Pigment from Bacillus cereus M¬¬¬1¬¬16 (MTCC5521): Production Parameters and Antibacterial Activity. Appl. Biochem. Biotechnol. 164: 767-779

Barnett, T.A., Hageman, J. 2011. Characterization of a brown pigment from Bacillus subtilis cultures. Canadian Journal of Microbiology . 29 (3): 309-315

Khaneja, R., Perez-Fons, L., Fakhry, S., Baccigalupi, L., Steiger, S, To, E., Sandmann, G., Dong, T.C., Ricca, E., Fraser, P.D., Cutting, S.M. 2010. Carotenoids found in Bacillus. Journal of Applied Microbiology. 108: 1889-1902

Fakhry, S.S, Sorrentini, I., Ricca, E., De Felice, M., Baccigalupi, L. 2008. Characterization of Spore Forming Bacilli Isolated From the Human Gastrointestinal Tract. J. Appl. Microbiol . 105(6):2178-86

Fakhry, S.S., Hasana, M.A., Hashim, S.T., Jebur, Z.A., Abdulhameed, F.F. 2017. Stress Responses in Pigmented Bacillus Pumilus SF214. Jurnal Teknologi (Sciences & Engineering). 79 (5): 197–201.

Barbosa, Tm., Serra, Cr., LaRagione, RM., Woodward, MJ., Henriques, AO. 2005. Screening for Bacillus isolates in the Broiler Gastrointestinal Tract. Applied Environmental Microbiology. &1 (2): 968- 978

Cuting , S.M., Vavder-Horn, P.B. 1999. Genetic analysis in Molecular Biological Methods for Bacillus ed. Harwood, C.R. and Cutting, S.M.PP,-27 74. Chichester, England: John Wiley& Sons Ltd.

Green, D., Wakelen,P., Page, A., Barnes, A., Baccigalupi, L., Ricca,E and Cutting, S. 1999. Characterization of two Bacillus probiotics species .Appl Environ Microbiol. 65. 4288-4291.

Bachvarov, DR., Ivanov, IG. 1983. Large Scale Purification of Plasmid DNA. Preparative Biochemistry. 13(2): 161-166

Sastry, KP., Kumar, RR., Kumar, AN., Sneha, G., Elizabeth, M. 2012. Morpho-chemical Description and Antimicrobial activity of Different Ocimum species. Journal of plant Development. 19: 53-64

Mendenhall, M.K., Ford, S.L., Emerson, C.L., Wells, R.A., Gines, L.G., Eriks, I.S. 2000. Detection and differentiation of Mycobacterium avium and Mycobacterium genavense by polymerase chain reaction and restriction enzyme digestion analysis. J Vet Diagn Invest 12:57–60

Casjens, S., Huang, WM. 1982. Initiation of sequential packaging of bacteriophage P22 DNA. Journal of Molecular Biology.157 (2): 287-298.

Nucleic acid cleanup In Nucleic Acid Sample Preparation for Downstream Analyses. Principles and Methods in GE Healthcare life science Hand book. Chapter 8 pp. 105-110 available on line https://cdn.gelifesciences.com/dmm3bwsv3/AssetStream.aspx?mediaformatid=10061&destinationid=10016&assetid=14455

Dubanau, DA. 1982. Genetic transformation in Bacillus subtilis. In: The molecular biology of the Bacilli, DA. Dubnau, ed., Academic Press, New York, 1: 148–175

Nakano,M. M., Dailly, Y. P., Zubber, P., Clark, and D. P. (1997) Characterization of anaerobic fermentative growth of Bacillus subtilis: identification of fermentation end products and genes required for growth. J Bacteriol. 179 (21):6749-55.

Reichelt, JL., Baumann, P. 1974. Effect of Sodium Chloride on Growth of Heterotrophic Marine Bacteria.Arch Microbiol. 97(4):329-45.

Sun, B., Zhang, XH., Tang, X., Wang, S., Zhong, Y., Chen, J., Austin, B. 2007. A Single Residue Change in Vibrio harveyHemolysinResultsin the Loss of Phospholipase and hemolytic Activities and Pathogenicity for Turbot (Scophthalmusmaximus). J Bacteriol. Mar; 189 (6):2575-9. Epub 12.

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Published

2022-03-29

Issue

Vol. 4 No. 2 (2021): Journal of Applied Sciences and Advanced Technology

Section

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