The Effectiveness of Green Grape Extract (Vitis vinifera) on Decreasing White Rat (Rattus novergicus) Triglycerides Levels

Article Sidebar

PDF
Published: Jun 2, 2022
DOI: https://doi.org/10.24853/mmj.3.1.1-9
Keywords:
dyslipidemia green grape extract triglyceride level

Main Article Content

Ketut Lilik Arwati
Department of Nutrition, Polytechnic of Health Denpasar, Bali, Indonesia
Ni Nyoman Astika Dewi
Department of Medical Laboratory Technology, Polytechnic of Health Denpasar, Bali, Indonesia
Heri Setiyo Bekti
Department of Medical Laboratory Technology, Polytechnic of Health Denpasar, Bali, Indonesia
Ni Wayan Rika Kumara Dewi
College of Health Sciences Bali Wisnu Dharma, Bali, Indonesia
Andi Udin Saransi
Faculty of Medicine, University of Udayana, Bali, Indonesia

Abstract

Background: Grapes (Vitis vinifera) are a source of antioxidants with high content of polyphenols and anthocyanins. Grape skin is rich in flavonoids. Anthocyanins and flavonoids have the effect of lowering blood triglyceride levels. Purposes: This study aimed to determine the effect of using green grape extract (GGE) in lowering triglyceride levels in the blood. Methods: Five groups of white male rats (Rattus novergicus) were divided into a negative control group (KN), positive control (KP), rats with a GGE dose of 100 mg/200g BW as P1, rats with a GGE dose of 250mg/200g BW as P2, and rats with GGE dose of 500 mg/200g BW as P3. Each group was given a hypercholesterolemic diet for five weeks. Groups P1, P2, and P3 were given GGE according to their respective doses for 14 days, then measured triglyceride levels in the blood. Results: The results showed that GGE 500mg/200g BW significantly reduced triglyceride levels in all treated mice. Conclusion: These results indicate that GGE has a great potential to treat dyslipidemia by lowering triglyceride levels in the blood.

Article Details

Issue
Vol. 3 No. 1 (2022): Muhammadiyah Medical Journal (MMJ)
Section
Articles

Authors who publish in the Muhammadiyah Medical Journal agree to the following terms:

  1. Authors retain copyright and grant Muhammadiyah Medical Journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution Licence that allows others to adapt (remix, transform, and build) upon the work non-commercially with an acknowledgement of the work's authorship and initial publication in Muhammadiyah Medical Journal.
  2. Authors are permitted to share (copy and redistribute) the journal's published version of the work non-commercially (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in Muhammadiyah Medical Journal.
Author Biographies

Ketut Lilik Arwati, Department of Nutrition, Polytechnic of Health Denpasar, Bali, Indonesia

Department of Nutrition, Polytechnic of Health Denpasar, Bali, Indonesia

Ni Nyoman Astika Dewi, Department of Medical Laboratory Technology, Polytechnic of Health Denpasar, Bali, Indonesia

Department of Medical Laboratory Technology, Polytechnic of Health Denpasar, Bali, Indonesia

Heri Setiyo Bekti, Department of Medical Laboratory Technology, Polytechnic of Health Denpasar, Bali, Indonesia

Department of Medical Laboratory Technology, Polytechnic of Health Denpasar, Bali, Indonesia

Ni Wayan Rika Kumara Dewi, College of Health Sciences Bali Wisnu Dharma, Bali, Indonesia

College of Health Sciences Bali Wisnu Dharma, Bali, Indonesia

Andi Udin Saransi, Faculty of Medicine, University of Udayana, Bali, Indonesia

Faculty of Medicine, University of Udayana, Bali, Indonesia

References

Nasution LS. Pengaruh Pemberian Likopen terhadap Perkembangan Lesi Aterosklerosis pada Tikus Hiperkolesterolemia. J Kedokt dan Kesehat. 2013;9(2).

Anwari IS, Sutysna H. The Effect of Olive Oil (Extra Virgine Olive Oil) and Moderate-Intensity Exercise On The Level of High Density Lipoprotein (HDL) in Male Wistar Strain Rat (Rattus novergicus L) Which Was Given High Fatty Diet. Muhammadiyah Med J. 2020;1(2) :69.

Rachmat S, Wiramihardja KK. Obesitas Permasalahan dan Terapi Praktis. Jakarta: Penerbit Sagung Seto; 2009.

Muchtadi D. Gizi Anti Penuaan Dini. Bandung: Alfabeta; 2008.

Kershaw EE, Flier JS. Adipose tissue as an endocrine organ. J Clin Endocrinol Metab. 2004 Jun;89(6): 2548–56.

Tsalissavrina I, Wahono D, Handayani D. Pengaruh Pemberian Diet Tinggi Karbohidrat Dibandingkan Diet Tinggi Lemak Terhadap Kadar Trigliserida dan HDL Darah. J Kedokt Brawijaya. 2006;22(2):80–9.

Hasibuan HPT, Thristy I. Comparison of Tryglicerides Levels and Total Cholesterol in Ischemic Stroke and Haemorrhagic Stroke Patients. Muhammadiyah Med J. 2020;1(2):49.

Aaronson PI, Jeremy PTW. Hiperlipidemia. In: At a Glance. 3rd ed. Jakarta; 2010. p. 78–9.

Jo S-P, Kim J-K, Lim Y-H. Antihyperlipidemic Effects of Rhapontin and Rhapontigenin from Rheum undulatum in Rats Fed a High-Cholesterol Diet. Planta Med. 2014 Aug 15;80(13):1067–71.

Ivorra MD, Payá M, Villar A. A review of natural products and plants as potential antidiabetic drugs. J Ethnopharmacol. 1989 Dec;27(3): 243–75.

Tarmizi. Buah Anggur Berpotensi Anti Kanker [Internet]. 2010 [cited 2015 Mar 8]. Available from: http://kimia.unp.ac.id/?p=221

Tapas A, Sakarkar D, Kakde R. Flavonoids as Nutraceuticals: A Review. Trop J Pharm Res. 2008; 7(3):1089–99.

Pugliese AG, Tomas-Barberan FA, Truchado P, Genovese MI. Flavonoids, proanthocyanidins, vitamin C, and antioxidant activity of Theobroma grandiflorum (Cupuassu) pulp and seeds. J Agric Food Chem. 2013 Mar;61(11):2720–8.

Nashriana N, Wirjatmadi B, Adriani M. Combined Food (Bekatul dan Lemak) Menurunkan Kadar Kolesterol Total, Trigliserida, dan LDL pada Tikus Galur Wistar Combined Food (Rice Bran and Fat) Reduce of the Total Cholesterol Levels, Triglycerides, and LDL of Wistar Strain Rats. J Kedokt Brawijaya. 2015;28(3):208.

Winarsi H. Produk Oksidasi pada Senyawa Lipid. Antioksidan Alami dan Radikal Bebas. Kanisius; 2007. 50–59 p.

Amr. Pterostilbene. Monograph. Altern Med Rev. 2010 Jul;15(2): 159–63.

McCormack D, McFadden D. A Review of Pterostilbene Antioxidant Activity and Disease Modification. Oxid Med Cell Longev. 2013;1–15.

Saputra F, Sutrisna E, Nurhayani N. Uji Efek Ekstrak Etanol 96% Anggur Merah (Vitis Vinifera) Terhadap Penurunan Kadar Trigliserida Pada Tikus Putih (Rattus Novergicus) Yang Diinduksi Triton X-100. Biomedika. 2017;8(2):31–8.

Supranto J. Teknik sampling untuk survey dan eksperimen. Jakarta: PT Rineka Cipta; 2000.

COBAS. Roche triglycerides Package Insert. 2019.

Rees DA, Alcolado JC. Animal models of diabetes mellitus. Diabet Med. 2005 Apr;22(4):359–70.

Tripani WJ, Ilmiawan MI, Handoko W. The Effect Of Single- And Repeated- Excessive Swimming Exercise On Kidney Histopathology Of Male Rats Wistar Strain (Rattus Norvegicus). Muhammadiyah Med J. 2020;1(1):33.

Lee M-J, Feliers D, Sataranatarajan K, Mariappan MM, Li M, Barnes JL, et al. Resveratrol ameliorates high glucose-induced protein synthesis in glomerular epithelial cells. Cell Signal. 2010 Jan;22(1):65–70.

Park S-H, Park T-S, Cha Y-S. Grape seed extract (Vitis vinifera) partially reverses high fat diet-induced obesity in C57BL/6J mice. Nutr Res Pract. 2008;2(4):227–33.

Orbayinah S, Permana KE. Pengaruh Ekstrak Buah Anggur Merah (Vitis vinifera L) terhadap Kadar Trigliserida Darah Tikus Putih (Rattus norvegicus). Mutiara Med. 2011;11(3):175–80.

Yokozawa T, Nakagawa T, Kitani K. Antioxidative activity of green tea polyphenol in cholesterol-fed rats. J Agric Food Chem. 2002 Jun;50(12):3549–52.

Lamson DW, Brignall MS. Antioxidants and cancer, part 3: quercetin. Altern Med Rev. 2000 Jun;5(3):196–208.

Khakim. Ketoksikan akut ekstrak air daun benalu (Dendrophthoe pentandra (L.) Miq. dan Dendrophthoe falcata (L.f). Ertingsh) pada mencit jantan dan uji kandungan kimia. Universitas Gadjah Mada; 2000.