Protective Effect of Combination Black Seed Oil (Nigella sativa) and Honey on the Duodenum of Rats Exposed to Cisplatin
Abstract
Background: Cisplatin (CP) is an anticancer agent with various side effects, including duodenal mucosal damage. It is well known that black seed oil (BSO) and honey (H) are rich in antioxidants and have a mucosal protective effect. Purposes: To determine the protective effect of the BSO and H combination on the histopathology of duodenal rats given CP exposure. Methods: Randomized post-test-only control group design was used in this study. A total of 30 rats were divided into ten groups, namely the control group (K), treatment groups (P1-P8), and cisplatin group (C). The treatment groups were given BSO and H orally for 21 days, and CP was given intraperitoneally on day 18 to the treatment and cisplatin groups. On day 22, the duodenal tissue was taken for preparation and histopathological assessment. Data analysis using IBM SPSS v.24 and Compusyn program. Result: There were significant differences in the duodenal mucosa damage scores of the P1-P8 group compared to the C group (p<0.05). The combination index of P7 exerted a synergism effect (CI < 1). Conclusion: The combination of BSO and H exerted a protective effect on the histopathological of rats’ duodenal tissue induced with CP, and the combination of BSO 2 mL/KgBW and H 3.7 mL/KgBW exerted a synergism effect.
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Siddiq AM, Ilmiawan MI, Handini M. Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats. Muhammadiyah Med J. 2020;1(2):43.
Yu W, Chen Y, Dubrulle J, Stossi F, Putluri V, Sreekumar A, et al. Cisplatin generates oxidative stress which is accompanied by rapid shifts in central carbon metabolism. Sci Rep. 2018;8(4306).
Gao J, Liu Y, Piao Y. Curcumin treatment attenuates cisplatin-induced gastric mucosal inflammation and apoptosis through the NF- κ B and MAPKs signaling pathway. Hum Exp Toxicol. 2022;41.
Qian W, Nishikawa M, Haque AM, Hirose M, Mashimo M, Sato E et al. Mitochondrial density determines the cellular sensitivity to cisplatin-induced cell death. Am J Physiol Cell Physiol. 2005;289(6):C1466-75.
Noviana R, Ilmiawan MI, Handini M. Synergistic Protective Effect of Commercial Nigella Sativa Oil and Honey Combination against Cisplatin-induced Nephrotoxicity in Rats Efek Protektif Sinergis Kombinasi Minyak Jintan Hitam ( Nigella sativa ) Komersial dan Madu terhadap Nefrotoksisitas pada. J Biotek Medisiana Indones. 2020;9(1):57–66.
Ahmad F, Ali F, Amir S, Saad HH, Wahab S, Idreesh M, et al. An updated knowledge of Black seed (Nigella sativa Linn.): Review of phytochemical constituents and pharmacological properties. J Herb Med. 2020;25(January):1–11.
Samarghandian S, Farkhondeh T, Samini F. Honey and health: A review of recent clinical research. Pharmacognosy Res. 2017;9(2):121–7.
Shahid F, Farooqui Z, Khan AA, Khan F. Oral Nigella sativa oil and thymoquinone administration ameliorates the effect of long-term cisplatin treatment on the enzymes of carbohydrate metabolism, brush border membrane, and antioxidant defense in rat intestine. Naunyn Schmiedebergs Arch Pharmacol. 2018;391:145–57.
Alkadri SLF, Ilmiawan MI, Handini M. Efek Protektif Kombinasi Minyak Jintan Hitam dan Madu terhadap Hepatotoksisitas pada Tikus Akibat Sisplatin. eJKI. 2019;7(2):101–8.
Underwood. J.C.E. General and Systematic Pathology. 6th ed. Churchill Livingstone: Elsevier; 2013.
Yamamoto H, Ishihara K, Takeda Y, Koizumi W IT. Changes in the mucus barrier during cisplatin-induced intestinal mucositis in rats. Biomed Res Int. 2013;2013:276186.
Arivarasu NA, Priyamvada S MR. Oral administration of caffeic acid ameliorates the effect of cisplatin on brush border membrane enzymes and antioxidant system in rat intestine. Exp Toxicol Pathol. 2013;65(1–2):21–5.
Kumar V, Cotran R, Robbins S. Buku Ajar Patologi. 9th ed. Jakarta: EGC; 2015.
Briga M, Odobasic A, Sestan I, Begic S. Black Seed Oils as an Additive to Honey. Eur J Accounting, Audit Financ Res. 2019;7(1):15–25.
Egilmez OK, Kokten N, Kalcioglu MT, Dogan Ekici AI, Serifler S, Yesilada E. Investigation of the Protective Effect of Nigella Sativa Oil in Cisplatin Induced Oral Mucositis: An Experimental Study. Turkish Arch Otorhinolaryngol. 2020;58(1):10–5.
Ibrahim A, Eldaim MAA A-DM. Nephroprotective effect of bee honey and royal jelly against subchronic cisplatin toxicity in rats. Cytotechnology. 2016;68(4):1039–48.
Mohamed M, Sulaiman SA, Jaafar H SK. Antioxidant protective effect of honey in cigarette smoke-induced testicular damage in rats. Int J Mol Sci. 2011;12(9):5508–21.
Kamilatussaniah, Yuniastuti A IR. Pengaruh suplementasi madu kelengkeng terhadap kadar TSA dan MDA tikus putih yang diinduksi timbal (Pb). J MIPA. 2015;38(2):108–14.
Chikrista K, Ilmiawan MI, Handini M. Efek protektif kombinasi minyak jintan hitam (Nigella sativa) dan madu pada ginjal tikus yang diberi pajanan cisplatin. J Cerebellum. 2021;6(4):115.
EL-Kholy WM, Hassan HA, Nour SE, Elmageed ZEA MK. Hepatoprotective effects of Nigella sativa and bees’ honey on hepatotoxicity induced by administration of sodium nitrite and sunset yellow. FASEB J. 2009;1(23):733.2-733.2.
DOI: https://doi.org/10.24853/mmj.4.1.8-14
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