Kadar Neuroglobin dan Sitoglobin dalam Plasma, Cairan Serebro Spinalis, dan Jaringan Otak Pasien Strok Hemoragik

Ninik Mudjihartini, Mohamad Saekhu, Sri Widia A. Jusman, Mohamad Sadikin

Abstract


Latar belakang: Otak memerlukan oksigen yang banyak selain glukosa. Hipoksia iskemik karena strok hemoragik atau Spontaneous intracerebral hemorrhage (sICH) dapat mengganggu suplai oksigen dan nutrisi ke otak berakibat produksi energi di otak akan menurun. Deplesi energi ini menyebabkan kerusakan dan kematian sel otak terjadi lebih cepat. Neuroglobin (Ngb) dan sitoglobin (Cygb) merupakan protein golongan globin yang terdapat di otak dan berperan sebagai protein pengikat oksigen di mitokondria. Tujuan: Penelitian ini bertujuan mendapatkan gambaran/profil kadar Ngb dan Cygb, di plasma, cairan serebro spinal (CSS), dan jaringan otak pasien strok hemoragik. Metode: Penelitian ini merupakan penelitian lanjutan menggunakan sampel plasma, CSS, dan jaringan otak yang diperoleh saat kraniotomi evakuasi hematoma pasien strok hemoragik sICH di rumah sakit Cipto Mangunkusumo dan rumah sakit lainnya di Jakarta. Kadar protein Ngb dan Cygb dari plasma, CSS, dan jaringan otak diukur dengan metode ELISA. Hasil: Rerata kadar Ngb otak adalah 0,058 ng/mg protein otak, sedangkan di plasma dan CSS masing-masing adalah 0,017 ng/mg protein otak dan 0,013 ng/mg protein otak atau 29,31% dan 22,41% dari rerata kadar Ngb otak. Rerata kadar Cygb otak adalah 4,943 ng/mg protein otak, di CSS adalah 1,685 ng/mg protein otak, atau 25,26% dari rerata Cygb otak, sedangkan di dalam plasma hampir tidak terdeteksi. Simpulan: Pada keadaan hipoksia oleh karena strok hemoragik sICH, protein Ngb dan Cygb dapat diukur di plasma, CSS, dan jaringan otaknya.


Keywords


neuroglobin; sitoglobin; strok hemoragik

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References


Patil M, Patel S. Pharmacogenomic analysis of single-nucleotide polymorphisms of angiotensin-I converting enzyme gene in stroke. Asian J Pharm Res. 2015 Jan 1;8:308–10.

Qureshi AI, Tuhrim S, Broderick JP, Batjer HH, Hondo H, Hanley DF. Spontaneous intracerebral hemorrhage. N Engl J Med. 2001 May;344(19):1450–60.

Feigin VL, Lawes CMM, Bennett DA, Barker-Collo SL, Parag V. Worldwide stroke incidence and early case fatality reported in 56 population-based studies: a systematic review. Lancet Neurol. 2009 Apr;8(4):355–69.

Feigin VL, Krishnamurthi R V., Parmar P, Norrving B, Mensah GA, Bennett DA, et al. Update on the global burden of ischemic and hemorrhagic stroke in 1990-2013: The GBD 2013 study. Neuroepidemiology. 2015;45(3) :161–76.

Feigin VL, Forouzanfar MH, Krishnamurthi R, Mensah GA, Connor M, Bennett DA, et al. Global and regional burden of stroke during 1990-2010: findings from the Global Burden of Disease Study 2010. Lancet (London, England) [Internet]. 2014 Jan 18;383(9913): 245–54. Available from: https:// pubmed.ncbi.nlm.nih.gov/24449944

van Asch CJ, Luitse MJ, Rinkel GJ, van der Tweel I, Algra A, Klijn CJ. Incidence, case fatality, and functional outcome of intracerebral haemorrhage over time, according to age, sex, and ethnic origin: a systematic review and meta-analysis. Lancet Neurol. 2010 Feb;9(2):167–76.

Broderick JP, Brott TG, Duldner JE, Tomsick T, Huster G. Volume of intracerebral hemorrhage. A powerful and easy-to-use predictor of 30-day mortality. Stroke. 1993 Jul;24(7):987–93.

Hemphill JC, Greenberg SM, Anderson CS, Becker K, Bendok BR, Cushman M, et al. Guidelines for the Management of Spontaneous Intracerebral Hemorrhage: A Guideline for Healthcare Professionals from the American Heart Association/American Stroke Association. Stroke. 2015;46(7): 2032–60.

Steiner T, Al-Shahi Salman R, Beer R, Christensen H, Cordonnier C, Csiba L, et al. European Stroke Organisation (ESO) guidelines for the management of spontaneous intracerebral hemorrhage. Int J stroke Off J Int Stroke Soc. 2014 Oct;9(7):840–55.

De Marinis E, Fiocchetti M, Acconcia F, Ascenzi P, Marino M. Neuroglobin upregulation induced by 17β-estradiol sequesters cytocrome c in the mitochondria preventing H2O2-induced apoptosis of neuroblastoma cells. Cell Death Dis. 2013 Feb;4(2):e508.

Götting M, Nikinmaa M. More than hemoglobin - the unexpected diversity of globins in vertebrate red blood cells. Physiol Rep [Internet]. 2015 Feb 3;3(2):e12284. Available from: https://pubmed.ncbi.nlm.nih.gov/25649247

Burmester T, Weich B, Reinhardt S, Hankeln T. A vertebrate globin expressed in the brain. Nature [Internet]. 2000;407(6803):520–3. Available from: https://doi.org/ 10.1038/35035093

Ascenzi P, Bocedi A, de Sanctis D, Pesce A, Bolognesi M, Marden MC, et al. Neuroglobin and cytoglobin: Two new entries in the hemoglobin superfamily*. Biochem Mol Biol Educ a Bimon Publ Int Union Biochem Mol Biol. 2004 Sep;32(5):305–13.

Mitz SA, Reuss S, Folkow LP, Blix AS, Ramirez J-M, Hankeln T, et al. When the brain goes diving: glial oxidative metabolism may confer hypoxia tolerance to the seal brain. Neuroscience. 2009 Oct;163(2): 552–60.

Kawada N, Kristensen DB, Asahina K, Nakatani K, Minamiyama Y, Seki S, et al. Characterization of a stellate cell activation-associated protein (STAP) with peroxidase activity found in rat hepatic stellate cells. J Biol Chem. 2001 Jul;276(27): 25318–23.

Kawada N. Cytoglobin as a Marker of Hepatic Stellate Cell-derived Myofibroblasts. Front Physiol. 2015;6:329.

Hundahl CA, Allen GC, Hannibal J, Kjaer K, Rehfeld JF, Dewilde S, et al. Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain. Brain Res. 2010 May;1331: 58–73.

Paulbabu K, Deepak Singh K, Prashanti P, Padmaja M. Neuroprotective potential and efficacy of neurodegenerative disorders of fruit extract of Aegle marmelos. Int J Pharm Pharm Sci. 2015;7(1):155–9.

Dirnagl U, Iadecola C, Moskowitz MA. Pathobiology of ischaemic stroke: an integrated view. Trends Neurosci. 1999 Sep;22(9):391–7.

Yu Z, Liu N, Liu J, Yang K, Wang X. Neuroglobin, a novel target for endogenous neuroprotection against stroke and neurodegenerative disorders. Int J Mol Sci [Internet]. 2012/06/07. 2012;13(6):6995–7014. Available from: https://pubmed. ncbi.nlm.nih.gov/22837676

Hankeln T, Ebner B, Fuchs C, Gerlach F, Haberkamp M, Laufs TL, et al. Neuroglobin and cytoglobin in search of their role in the vertebrate globin family. J Inorg Biochem. 2005 Jan;99(1):110–9.

Xie LK, Yang SH. Brain globins in physiology and pathology. Med Gas Res. 2016;6(3):154–63.

Mudjihartini N, Lasma Nurhayati, Saekhu M, Sri Widia A Jusman, Jan Purba, Mohamad Sadikin. Responses of brain tissues against hypoxic condition in hemorrhagic stroke patients: Neuroglobin expression in brain tissue and plasma. Asian J Pharm Clin Res [Internet]. 2017 Feb 1;10(2 SE-Original Article(s)):407–9. Available from: https://www. innovareacademics.in/journals/index.php/ajpcr/article/view/15971

Warburg O, Christian W. Isolierung und Kristallisation des Gärungsferments Enolase. Naturwissenschaften [Internet]. 1941;29(39):589–90. Available from: https://doi.org/10.1007/ BF01482279

Baez E, Echeverria V, Cabezas R, Ávila-Rodriguez M, Garcia-Segura LM, Barreto GE. Protection by Neuroglobin Expression in Brain Pathologies [Internet]. Vol. 7, Frontiers in Neurology . 2016. Available from: https://www. frontiersin.org/article/10.3389/fneur.2016.00146

Cai H, Zheng S, Cai B, Yao P, Ding C, Chen F, et al. Neuroglobin as a Novel Biomarker for Predicting Poor Outcomes in Aneurysmal Subarachnoid Hemorrhage. World Neurosurg. 2018 Aug;116:e258–65.

Gao Y, Wang B, Miao Y, Han Y. Serum Neuroglobin as a Potential Prognostic Biomarker for Cognitive Impairment After Intracerebral Hemorrhage [Internet]. Vol. 13, Frontiers in Neurology . 2022. Available from: https://www. frontiersin.org/article/10.3389/fneur.2022.885323

Casado B, Pannell LK, Whalen G, Clauw DJ, Baraniuk JN. Human neuroglobin protein in cerebrospinal fluid. Proteome Sci [Internet]. 2005;3(1):2. Available from: https:// doi.org/10.1186/1477-5956-3-2

Fordel E, Geuens E, Dewilde S, Rottiers P, Carmeliet P, Grooten J, et al. Cytoglobin expression is upregulated in all tissues upon hypoxia: an in vitro and in vivo study by quantitative real-time PCR. Biochem Biophys Res Commun. 2004 Jun;319(2):342–8.

Li RC, Lee SK, Pouranfar F, Brittian KR, Clair HB, Row BW, et al. Hypoxia differentially regulates the expression of neuroglobin and cytoglobin in rat brain. Brain Res. 2006 Jun;1096(1):173–9.




DOI: https://doi.org/10.24853/mujg.3.1.1-8

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