ANALISIS KERAGAMAN DAN KEKERABATAN KACANG TUNGGAK (Vigna unguiculata L) GENERASI M2

Yukarie Ayu Wulandari, Sobir Sobir, Syarifah Iis Aisyah

Abstract


Cowpea (V. unguiculata L) has great potential as a nutritious food as a substitute for soybeans because it contains sufficient protein and low fat content. The diversity of cowpea is low so that need to increase diversity through the mutation induction of gamma ray irradiation. The study was carried out in the experimental garden of Pasir Kuda PKHT of IPB in February - May 2018 using a design of augmented in the Complete Group Design in a Randomized. The study was carried out using 90 putative mutant genotypes of M2 generation as the test genotype and KM4 genotype as a comparison which was repeated 10 times. The results showed that the M2 generation putative mutants showed diversity in the qualitative and quantitative characters of cowpea. High genetic diversity is shown in the character of plant height, harvest period, number of seeds / pods and weight of cowpea seeds / plants and high broad mean heritability values obtained on the character of stem length, flowering age, number of seeds / pods and weight of beans / plant nuts arrears. The result of kinship analysis showed thirteen different putative mutant genotypes with KM4 genotypes, namely T6599P, T8028P, T7525P, T7551P, T7520P, T6574P, T6533P, T7058P, T6577P, T6591P, T7062P, T7069P and T6561.


Keywords


gamma ray irradiation, heritability, kinship, putative mutant

Full Text:

PDF

References


Adisarwanto T, Riwanodja, Suhartina. 1998. Budi daya tanaman kacang tunggak. Monograf BALITKABI 3: 78-83.

[BKPPP] Badan Ketahanan Pangan dan Pelaksana Penyuluhan. 2014. Data Kandungan Gizi Bahan Pangan dan Olahan Golongan Kacang-kacangan dan Biji-bijian. Yogyakarta (ID): BKPPP Bantul.

Chavan JK, Kadam SS, Salunkhe DK. 1989. Cowpea. In Salunkhe DK, Kadam SS (Eds) CRC Handbook of World Food Legumes: Nutritional Chemistry, Processing Technology and Utilization, vol 2. CRC Press, Inc. Boca Raton, Florida : 1-22

Federer WT. 1994. Augmented Experiment Designs with Recofery of Interblock and Intervariety Information. Itacha (US): Cornell University.

Hartati S, Darsana L. 2015. Karakterisasi anggrek alam secara morfologi dalam rangka pelestarian plasma nutfah. J. Agron Indonesia. 43 (2): 133–139.

Herawati R, Purwoko BS, Dewi IS. 2009. Keragaman genetik dan karakter agronomi galur haploid ganda padi gogo dengan sifat-sifat tipe baru hasil kultur antera. J Agron Indonesia. 37(2):87-94.

Herison K, Rustikawati, Sutjahjo SH, Aisyah SI. 2008. Induksi mutasi melalui iradiasi sinar gamma terhadap benih untuk meningkatkan keragaman populasi dasar Jagung (Zea mays L). J Akta Agrosia 11(1): 57-62.

Human S. 2003. Peran iptek nuklir dalam pemuliaan tanaman untuk mendukung industri pertanian. Prosiding Pertemuan dan Presentasi Ilmiah Penelitian Dasar Ilmu Pengetahuan dan Teknologi Nuklir P3TM BATAN. Yogyakarta.

[IAEA] International Atomic Energy Agency. 2003. Mutation Breeding News Letter No.46. IAEA, Vienna, Austria.

Ishak. 2012. Sifat agronomi, heritabilitas dan interaksi G x E galur mutan padi gogo (Oryza sativa L.). J. Agron. Indonesia 40:105-111.

Noorrohmah, Sobir, Efendi D. 2015. Analisis keragaman genetik manggis dalam satupohon. J. Hort. 25(2):106-112.

Nura, Syukur M, Khumaida N, Widodo. 2015. Radiosensitivitas dan heritabilitas ketahanan terhadap penyakit antraknosa pada tiga populasi cabai yang diinduksi iradiasi sinar gamma. J. Agron. Indonesia 43(3): 201-206.

Rif‘atunidaudina R. 2018. Kajian Keanekaragaman Morfologi, Metabolit Primer dan Molekuler Sumber Daya Genetik Kacang-kacangan Underutilized Indonesia. Tesis. Institut Pertanian Bogor.

Rosida EF, Hardiyanti Q, Murtiningsih. 2013. Kajian dampak substitusi kacang tunggak pada kualitas fisik dan kimia tahu. J. Tek. Pangan 5(2):138-149.

Sakin MA, Yildrim A, Gokmen S. 2004. The evaluation of agronomic traits of durum wheat (Triticum durum Desf.) mutants. Pak J. Biol Sci. 7(4):571-576.

Sigh S, Pradhan SK, Virk P. 2008. Genetic divergence in new plant type rice under shallow lowland ecosistem. SABRAO 40(1):1-8.

Singh RK, Chaudhary BD. 1979. Biometrical Methods in Quantitative Genetic Analysis. New Delhi (IN): Ludhiana Kalyani Pub.

Suhartina, Hapsari RT, Purwantoro. 2016. Keragaman plasma nutfah kedelai berdasarkan keragaan karakter morfo-agronomis. Bul. Plasma Nutfah 22(2): 109-118.

Syahril M. 2018. Rancangan bersekat (augmented design) untuk penelitian bidang pemuliaan tanaman. J. Penelitian 5(1): 63-66.

Syukur M, Sujiprihati S, Siregar A. 2010. Pendugaan parameter genetik beberapa karakter agronomi cabai F4 dan evaluasi daya hasilnya menggunakan rancangan perbesaran (Augmented design). J. Agrotropika 15:9-16.

Wani MR, Khan S. 2006. Estimates of genetic variability in mutated populations and the scope of selection for yield attributes in Vigna radiata (L.) Wilczek. Egypt J. Biol. 8: 1-6.

Yunianti R, Sastrosumarjo S, Sujiprihati S, Surahman M, Hidayat SH. 2010. Kriteria seleksi untuk perakitan varietas cabai tahan Phytophthora capsici Leonian. J. Agron. Indonesia 38:122




DOI: https://doi.org/10.24853/jat.5.1.46-56

Refbacks

  • There are currently no refbacks.


Copyright of Jurnal AGROSAINS dan TEKNOLOGI (e-ISSN:2528-3278, p-ISSN:2528-0201).

   Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Powered by Puskom-UMJ