SINTESA ASAM LAKTAT BERBAHAN BAKU TANDAN KOSONG KELAPA SAWIT MENGGUNAKAN TRICHODERMA RESEEI DAN LACTOBACILLUS ACIDIPILLUS
Abstract
Tandan kosong kelapa sawit (TKKS) merupakan limbah pertanian dengan kadar selulosa yang tinggi, sehingga berpotensi untuk dikonversi menjadi berbagai macam produk. Salah satu senyawa kimia yang dapat dihasilkan dari selulosa TKKS adalah asam laktat. Asam laktat merupakan bahan baku utama dalam pembuatan polimer biodegradable berupa polilactic acid (PLA). Penelitian ini bertujuan untuk mendapatkan konsentrasi mikroorganisme Lactobacillus acidophilus yang dapat menghasilkan asam laktat dengan konsentrasi yang tinggi. Tahapan penelitian meliputi pretreatment, hidrolisis dan fermentasi. Pretreatment TKKS menggunakan NaOH, Tahap hidrolisis selama 48 jam menggunakan Trichoderma reesei 5 % (w/w) dan tahap fermentasi selama 48 jam menggunakan Lactobacillus acidophilus dengan variasi 0.5; 1; 3; 5%. Analisa glukosa menggunakan spektrofotometri dan asam laktat menggunakan High performance liquid chromatography (HPLC). Konsentrasi gula reduksi yang dihasilkan dari proses hidrolisis sebesar 16,6-17,9 g/L dan konsentrasi asam laktat tertinggi 0.568 g/L didapatkan dengan penambahan Lactobacillus acidophilus 3%
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