Alpha Lipoic Acid Effect on Collagen of Extra Cellular Matrix β Cell Pancreas in Type 2 Diabetic Rats
Abstract
Kelainan pulau Langerhans (isletopathy) merupakan salah satu komplikasi DM tipe 2. Penelitian mengenai efek asam alfa lipoat (ALA) terhadap matriks ekstraseluler pankreas (ECM) pada DM tipe 2 masih terbatas. Penelitian ini bertujuan untuk mengetahui efek ALA terhadap ketebalan kolagen ECM sel β pankreas pada tikus diabetes melitus tipe 2. Penelitian eksperimental dengan design post test only control group design ini menggunakan 15 ekor tikus Rattus novergicus galur Wistar jantan yang dibagi menjadi tiga kelompok, yaitu kelompok kontrol, kelompok diabetes melitus tipe 2, dan kelompok diabetes melitus tipe 2 yang diberi ALA. Induksi diabetes melitus tipe 2 dilakukan dengan pemberian streptozotosin (50 mg/kgBB) diikuti nikotinamid (110 mg/kgBB) intraperitoneal. Dosis ALA yang digunakan 60 mg/kgBB/hari diberikan selama 3 minggu. Ketebalan kolagen matriks ekstraseluler sel β pankreas dinilai dengan pewarnaan Verhoeff's van Gieson (VvG) dengan sistem skoring. Hasil penelitian menunjukkan ketebalan kolagen matriks ekstraseluler pulau Langerhans pankreas tetap normal pada semua kelompok penelitian. Pemberian ALA 60 mg/kgbb selama 3 minggu pada tikus DM tipe 2 tidak memiliki efek terhadap ketebalan kolagen ECM pankreas. Dengan demikian perlu dilakukan penelitian lanjutan mengenai efek ALA terhadap kolagen matriks ekstra seluler pankreas DM tipe 2 tahap lanjut.
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DOI: https://doi.org/10.24853/jkk.16.1.68-75
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