Isolasi DNA pada Produk Otak-Otak Ikan Bandeng

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Published: Nov 16, 2022
DOI: https://doi.org/10.24853/mjnf.3.1.36-41
Keywords:
analisis DNA ekstraksi konsentrasi makanan

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Sri Utaminingsih
Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia
Sofia Dyah Utami
Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia
Alfi Sophian
Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia
http://orcid.org/0000-0002-5206-2110

Abstract

Latar belakang: Analisis mutu DNA hasil isolasi merupakan teknik analisis yang digunakan untuk mengetahui kualitas DNA hasil isolasi sehingga meminimalkan kegagalan proses amplifikasi dalam analisis molekuler. Tujuan: Tujuan dilakukan penelitian ini adalah untuk melihat mutu DNA hasil isolasi pada produk pangan olahan ikan berupa otak-otak ikan bandeng.  Prosedur ekstraksi DNA mengikuti protokol ekstraksi manual kit yang digunakan. Analisis mutu DNA hasil isolasi diukur menggunakan nanophotometer, dimana analisis mutu berdasarkan nilai konsentrasi dan kemurnian DNA hasil isolasi. Analisis data dilakukan menggunakan uji rata-rata nilai konsentrasi dan kemurnian. Hasil: Berdasarkan hasil analisis mutu DNA diperoleh hasil konsentrasi DNA berada pada kisaran 9.6 – 18.5 dengan nilai rata-rata DNA hasil isolasi berada pada nilai 13.4 ng/µL, sedangkan nilai kemurnian yang dibaca pada panjang gelombang A260/A28 berada pada kisaran 1.64 – 1.87, dengan nilai rata-rata berada pada nilai 1.79. Simpulan: Sehingga dapat disimpulkan bahwa DNA hasil isolasi yang dilakukan pada sampel produk pangan olahan otak-otak ikan bandeng menunjukkan nilai konsentrasi dan kemurnian yang masuk dalam kategori hasil isolasi DNA yang baik.

Article Details

Issue
Vol. 3 No. 1 (2022): Muhammadiyah Journal of Nutrition and Food Science (MJNF)
Section
Articles

(c) 2020 MJNF

Author Biographies

Sri Utaminingsih, Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia

Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia

Sofia Dyah Utami, Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia

Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia

Alfi Sophian, Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia

Pusat Pengembangan Pengujian Obat dan Makanan Nasional, Badan Pengawas Obat dan Makanan, Jakarta, Indonesia

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