Analisis Nilai Kemurnian DNA Menggunakan Nano Fotometer pada Rasio 260/230 yang Diisolasi dari Produk Nugget
DOI:
https://doi.org/10.24853/mjnf.3.2.82-86Keywords:
analisis, DNA, kemurnian, rasio, validasiAbstract
Latar belakang: Parameter nilai kemurnian yang dianalisis dari panjang gelombang dengan rasio 260/230 merupakan parameter validasi sekunder yang digunakan untuk melakukan analisis mutu DNA hasil isolasi pada produk nugget ayam. Tujuan: Tujuan dari dilakukannya penelitian ini adalah untuk memberi informasi tentang nilai analisis kemurnian DNA hasil isolasi yang dibaca pada rasio 260/230. Dengan diperolehnya informasi dari hasil penelitian ini diharapkan dapat memberi manfaat terhadap penelitian sejenis yang relevan. Metode: Metode yang digunakan dalam penelitian ini adalah metode isolasi DNA menggunakan teknik spin kolom, kemudian DNA hasil isolasi yang diperoleh dibaca menggunakan nano fotometer pada rasio 260/230. Hasil: Hasil pengukuran kemudian dihitung nilai rata-rata dan standar deviasinya. Berdasarkan hasil pengukuran DNA hasil isolasi diperoleh hasil kemurnian yang dibaca pada rasio A260/A230 berada pada kisaran 1,98 – 2,10, dengan nilai rata-rata berada pada nilai 2,043. Simpulan: Berdasarkan hasil tersebut maka dapat disimpulkan bahwa DNA hasil isolasi yang diperoleh menunjukkan kualitas DNA yang baik berdasarkan syarat mutu nilai kemurnian DNA pada rasio 260/230 yaitu pada kisaran 2,0 – 2,2.References
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